Incorporation of Phosphorus into Human Erythrocyte Ghost Fractions.
نویسندگان
چکیده
منابع مشابه
Incorporation of Fatty Acids into Phospholipids of Erythrocyte Membranes.
Incorporation of C14-labeled fatty acids into phospholipids by washed ghosts, prepared from human erythrocytes, required the addition of ATP and was markedly stimulated by MgC12 and coenzyme A. Labeled palmitic acid from l-C"-palmitoyl CoA was incorporated in the absence of added cofactors, The extent of incorporation of linoleic acid was greater than that of oleic, palmitic, or stearic acids. ...
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The phospholipid composition of human platelets and erythrocytes has been reported by several investigators (1-4) and the metabolism of phospholipids in human blood has been studied in some detail (5-9). Recent investigations of phospholipid metabolism of leukocytes have been reported (6, 10) but we are not aware of similar studies on platelets. In this laboratory, investigation of the biochemi...
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The endocytic vacuoles induced in white ghosts were found to be depleted of spectrin and therefore it was proposed that they arose from spectrin-free areas in the erythrocyte membrane . To follow changes in spectrin distribution during endocytosis, affinity-purified rabbit antispectrin antibodies were produced . Quantitative techniques were developed for the use of a highly specific '2'I-F(ab')...
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The osmotic swelling of intact erythrocytes in hypotonic solutions was measured using microhematocrit tubes, Van Allen tubes, and a calibrated Coulter counter. In agreement with earlier workers the intact cells did not behave as perfect osmometers, the cells swelling less than predicted by the Boyle-van't Hoff law. Erythrocyte ghosts were prepared from fresh intact erythrocytes by one-step hemo...
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Ceruloplasmin is a multicopper oxidase essential for normal iron homeostasis. To elucidate the mechanisms of copper incorporation into this protein, holoceruloplasmin biosynthesis was examined by immunoblot analysis and (64)Cu metabolic labeling of Chinese hamster ovary cells transfected with cDNAs encoding wild-type or mutant ceruloplasmin. This analysis reveals that the incorporation of coppe...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1964
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.18-2029